Movie 6.

High-resolution profile view of motile cilia from Dnai1-/- mNPEC. Representative high-resolution video of a profile view of motile cilia from Dnai1-/- murine nasopharyngeal epithelial cells scraped from cultures at a replay speed of 30 fr/s (0.1 x live) showing few active cilia with a lower CBF. Videos of scraped cells were taken by SAVA software on a Nikon Eclipse TE2000 inverted microscope with a 60X oil objective (NA = 1.4) with DIC-H optics and 2X post-objective magnification at 37°C and 5% CO2 with humidified air. Scale bar, 2 μm.

Lipid nanoparticle-encapsulated Dnai1 mRNA rescues ciliary activity in primary ciliary dyskinesia mouse cell models

Amanda J. Smith, Patrick R. Sears, Mirko Hennig, Rumpa B. Bhattacharjee, Weining Yin, Hannah Golliher, Daniella Ishimaru, T. Noelle Lombana, David J. Lockhart, Brandon A. Wustman, and Lawrence E. Ostrowski

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.264068