Movie 6.
High-resolution profile view of motile cilia from Dnai1-/- mNPEC. Representative high-resolution video of a profile view of motile cilia from Dnai1-/- murine nasopharyngeal epithelial cells scraped from cultures at a replay speed of 30 fr/s (0.1 x live) showing few active cilia with a lower CBF. Videos of scraped cells were taken by SAVA software on a Nikon Eclipse TE2000 inverted microscope with a 60X oil objective (NA = 1.4) with DIC-H optics and 2X post-objective magnification at 37°C and 5% CO2 with humidified air. Scale bar, 2 μm.
Lipid nanoparticle-encapsulated Dnai1 mRNA rescues ciliary activity in primary ciliary dyskinesia mouse cell models
J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.264068