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Movie 2.

Movement of reconstituted microtubule asters in the presence of HSET and ATP. GFP::CDK5RAP2 scaffolds (green) were incubated with BFP::γ-TuRC, HSET, ATP, and HiLyte647-labeled tubulin (red). As in Fig. 4F.

In vitro reconstitution of a minimal human centrosome scaffold capable of forming and clustering microtubule asters

Manolo U. Rios, Weronika E. Stachera, Nicole E. Familiari, Claudia Brito, Thomas Surrey, and Jeffrey B. Woodruff

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.264121