Movie 1.

Internalization of E-cadherin-tdTom after induction of oncogenic Ras by + Dox. Ras expression is marked by GFP. Representative timelapse video. Cells were imaged for up to 24hrs after Dox addition. Three independent fields of view are shown; the left panels display GFP fluorescence marking expression of Ras from the inducible GFP-P2A-Ras(Q61L) construct, and the right panels show E-cadherin-tdTom fluorescence. Images are maximum intensity projections from confocal slices generated on a Nikon A1R with 40x 1.2na oil objective. Note that GFP expression is not synchronized across the monolayers even though the cell line was clonally selected.