Movie 2.

Representative video of the inhibition of E-cadherin-tdTom internalization by treatment with Erlotinib (10 μM). Ras expression is marked by GFP. Cells were imaged for up to 24hrs after addition of ErloDnib and Dox. Three independent fields of view are shown; the left panels display GFP fluorescence marking expression of Ras from the inducible GFP-P2A-Ras(Q61L) construct, and the right panels show E-cadherin-tdTom fluorescence. Note that GFP expression is not synchronized across the monolayers even though the cell line was clonally selected.