Movie 1.

The video demonstrates the correlative imaging of AAV2 (green) in live-cell conditions and STORM imaging of detyrosinated tubulin (magenta). The real-time dynamics of AAV2 were observed one hour post-infection, with its trajectory displayed as a yellow track over the video, highlighting the movement of viral particles as they interact with the microtubule. AAV2 motility is notably restricted when encountering detyrosinated tubulin.

Suppressing microtubule detyrosination augments adeno-associated virus 2 endosomal escape and gene delivery

Shefali Tripathi, Shamshul Huda, Joydipta Kar, Dinesh Chandra, Giridhara R. Jayandharan, and Nitin Mohan

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.264190