Movie 2.

Random migration assay of Rap2-KO cells. MDA-MB-231 cells were seeded on fibronectin coated plates imaged with the 4x objective of an OLYMPUS IX83 inverted confocal microscope. Imaging lasted 12 hours with images taken every 20 minutes.

Ubiquitylation-dependent Rap2 activation regulates lamellipodia dynamics during cell migration

Andrew Neumann, Revathi Sampath, Emily Mayerhofer, Valeryia Mikalayeva, Vytenis Arvydas Skeberdis, Ieva SarapinienÄ—, and Rytis Prekeris

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.264375