Movie 6.

GFP-Rap2a-Q63E overexpression alters lamellipodia dynamics. MDA-MB-231 cells expressing GFP-Rap2a-Q63E were seeded on collagen coated plates and imaged with the 100x objective and 2.8x CSU-W1 T1 Super-Resolution spinning disk confocal SoRa mag changer disk using the 488 (left panel) and transmitted light (right panel) channels on a Nikon Ti2-E inverted microscope. Images were taken every 5 seconds for 5 minutes.

Ubiquitylation-dependent Rap2 activation regulates lamellipodia dynamics during cell migration

Andrew Neumann, Revathi Sampath, Emily Mayerhofer, Valeryia Mikalayeva, Vytenis Arvydas Skeberdis, Ieva SarapinienÄ—, and Rytis Prekeris

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.264375