Movie 2.

ΔF233 DHHC21 and STIM1 colocalize upon ER store depletion. DHHC21 KO cells were transfected with ΔF233 DHHC21-GFP and STIM1-mRFP, and TIRF microscopy was performed. Store depletion was induced using 10 μM thapsigargin (TG).