Movie 2.

Live imaging of buttoning in the spinal region of a control chick embryo. Supplemental movie 1 embryo was re-positioned to view the spinal region. The embryo is positioned so anterior is towards the “top”. Time in minutes shown in upper left corner. Scale bar: 100μm.

Increased tissue tension caused by depletion of CLDN3 in the non-neural ectoderm causes neural tube defects in chick embryos

Elizabeth-Ann Legere, Marie Dumont, Jordan A. Dias, Yojiro Yamanaka, Gabriel L. Galea, and Aimee K. Ryan

J Cell Sci 2026. 139:None-None; doi: 10.1242/jcs.264758