Movie 5.

Live-cell imaging of cdc13-sfGFPcp-24xMS2 with P.mad3-MCP-tdSG. Cells expressing MCP-tdSG from the mad3 promoter. Top: cells expressing cdc13-sfGFPcp-24xMS2; bottom: cells expressing cdc13-sfGFPcp (no MS2) as control. Z-stacks were acquired every 5 sec; a maximum intensity projection is shown. Note that both MCP-tdSG and Cdc13-sfGFPcp are visible in the green channel.