Movie 7.

Live-cell imaging of rad21-24xMS2 with P.mad3-MCP-tdSG. Cells expressing MCP-tdSG from the mad3 promoter as well as rad21-24xMS2. Z-stacks were acquired every 5 min; a maximum intensity projection is shown. The rad21 (cohesin) mRNA shows cell cycledependent expression just prior to septation and cell division.